Synergistic effect of vitamin D derivatives and retinoids on C2C12 skeletal muscle cells.
IUBMB Life
; 53(3): 175-81, 2002 Mar.
Article
em En
| MEDLINE
| ID: mdl-12102174
ABSTRACT
The response of C2C12 myoblasts to 1 nM 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], two vitamin D analogues (KH 1060 and EB 1089, which are 20-epi-22-oxa and 22,24-diene-analogues, respectively), 100 nM retinoids (9-cis retinoic acid, all-trans retinoic acid) and to combination treatments, after 72 h incubation, was studied. The incubation with 1,25(OH)2D3 was ineffective on either cell proliferation or [3H]thymidine incorporation (expressed as DPM per cell) or protein content per cell. On the contrary, all the other treatments inhibited cell proliferation, this inhibition being synergistic when the vitamin D derivatives were combined with 9-cis or all-trans retinoic acid, and increased [3H]thymidine incorporation and protein content per cell. The levels of the VDR protein remarkably increased in comparison with control cells, except for the incubation with 9-cis retinoic acid. This increase was particularly accentuated in C2C12 cells treated with KH 1060 and 9-cis retinoic acid in combination. These results, taken together, suggest a role for vitamin D derivatives and retinoids on C2C12 cells.
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Base de dados:
MEDLINE
Assunto principal:
Retinoides
/
Vitamina D
/
Calcitriol
/
Músculo Esquelético
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article