Calcium-induced Cytochrome c release from rat brain mitochondria is altered by digitonin.
Neurosci Lett
; 332(2): 91-4, 2002 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-12384218
ABSTRACT
To determine if calcium could release Cytochrome c (Cyt c) from brain mitochondria without activating the permeability transition (mPT), brain mitochondria were prepared in two different ways. Digitonin was used to lyse synaptosomes and release synaptosomal mitochondria or a Percoll gradient was used to separate non-synaptosomal mitochondria from the synaptosomes. In gradient-purified mitochondria, low levels of added digitonin produced swelling and Cyt c release. Digitonin augmented Ca(2+)-induced Cyt c release that was insensitive to the mPT inhibitors, cyclosporin A CsA and ADP. Similarly, in mitochondria prepared with digitonin, these inhibitors also failed to prevent Ca(2+)-induced Cyt c release. Thus the mPT-independent, Ca(2+)-induced Cyt c release pathway was attributable to alteration of the permeability properties of the outer mitochondrial membrane by digitonin.
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Base de dados:
MEDLINE
Assunto principal:
Química Encefálica
/
Cálcio
/
Grupo dos Citocromos c
/
Digitonina
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article