Disruption of a silencer domain by a retrotransposon.
Genetics
; 131(3): 519-29, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1321064
ABSTRACT
A galactose-inducible Ty element carrying the HIS3 gene has been used as an insertional mutagen to generate alpha-factor resistant mutants. This collection of Ty-induced mutations includes insertions into the gene for the alpha-factor receptor (STE2), several nonspecific STE genes, and mutations that lead to the expression of the normally silent HML alpha locus. The hml alpha "on" mutations fall into two classes, those that disrupt trans-acting regulators involved in silencing HML alpha and a novel class of mutations that activate HML alpha by insertion at that locus. The hml alphaTy "on" mutations illustrate the unusual ability of these retrotransposons to activate genes by overcoming gene silencing mechanisms. The hml alphaTy "on" mutations include examples of multimeric Ty arrays. Single Ty and solo delta insertion derivatives of these Ty multimers restore the ability of the silencing mechanism to repress HML alpha.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Fatores de Transcrição
/
Elementos de DNA Transponíveis
/
Regulação Fúngica da Expressão Gênica
/
Sequências Reguladoras de Ácido Nucleico
/
Receptores de Peptídeos
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article