A new, simple assay for long-chain acyl-CoA dehydrogenase in cultured skin fibroblasts using stable isotopes and GC-MS.
Biochim Biophys Acta
; 1180(1): 28-32, 1992 Oct 13.
Article
em En
| MEDLINE
| ID: mdl-1390941
ABSTRACT
In this paper, we present a new method for measurement of long-chain acyl-CoA dehydrogenase (LCAD) activities in cultured skin fibroblasts. The method is based upon gas chromatographic/mass spectrometric determination of 3-OH-hexadecanoic acid formed during incubation of fibroblasts in a medium containing palmitoyl-CoA and crotonase, to convert the enoyl-CoA ester produced into the 3-hydroxyacyl-CoA ester. The validity of the method is demonstrated by the finding of a full deficiency of LCAD in fibroblasts from three patients with an established deficiency of LCAD.
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Base de dados:
MEDLINE
Assunto principal:
Pele
/
Acil-CoA Desidrogenase de Cadeia Longa
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article