Colloidal gold immunostaining for the detection of platelet reactive allo- and autoantibodies. Use on fresh and frozen cells.
Haematologica
; 77(1): 89-91, 1992.
Article
em En
| MEDLINE
| ID: mdl-1398289
ABSTRACT
BACKGROUND:
Fresh washed platelets are commonly used as target cells for the detection of platelet reactive antibodies. The use of stored platelets would allow a faster execution of the tests and a rapid selection of phenotyped cells.METHODS:
Glass-adherent platelet monolayers were freeze stored. The antigen-antibody reaction between platelet antigens and platelet reactive allo- or autoantibodies was revealed by a modified antiglobulin test, using colloidal gold-labeled anti-human IgG. Immunogold staining (IGS) was compared with the platelet suspension immunofluorescence test (PSIFT) and with the solid-phase red cell adherence assay (SPRCA). The reactivity of fresh and freeze-stored platelets was compared.RESULTS:
IGS proved to be slightly less sensitive (10%) than PSIFT and SPRCA The results obtained with fresh and freeze-stored cells were scored identically.CONCLUSIONS:
The immunogold staining assay on freeze-stored platelets seems to be a convenient technical approach for platelet serology.
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Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
/
Plaquetas
/
Imuno-Histoquímica
/
Isoanticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article