Characterization of bacteria by multiparameter flow cytometry.
J Appl Bacteriol
; 73(5): 438-44, 1992 Nov.
Article
em En
| MEDLINE
| ID: mdl-1447060
ABSTRACT
An arc-lamp based flow cytometer was used to obtain high resolution measurements of the light scattering characteristics and DNA contents of eight different bacteria. Light scatter profiles of bacteria are a useful first step when flow cytometry is used to characterize organisms. Scanning and transmission electron microscopy of the bacterial samples demonstrate that the structural basis of the light scattering profiles is not always clear, i.e. some organisms appear to have anomalous light scattering characteristics. The use of a third measurement parameter, DNA content, allowed much better discrimination of the organisms. Flow cytometry shows great promise as a method for the rapid discrimination and identification of bacterial populations.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Técnicas de Tipagem Bacteriana
/
Citometria de Fluxo
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article