A new spectrophotometric assay for the determination of vitamin A and related compounds in serum.

ABSTRACT

A new, direct multicomponent spectrophotometric assay for determining the vitamin A, lycopene, the total beta-carotene+beta-cryptoxanthin, the total lutein+alpha-carotene content of human serum is reported. The new method has been validated by performing parallel HPLC assays and is likely to be especially useful for use in population screening programmes. It is also suitable for screening paediatric samples.