Quantification and molecular analysis of cathepsin D in breast cyst fluids.
Eur J Cancer
; 28A(4-5): 828-32, 1992.
Article
em En
| MEDLINE
| ID: mdl-1524902
ABSTRACT
Cyst fluids from 55 premenopausal women with gross cystic breast disease were classified by K+/Na+ ratio 19 with ratio over 1 (type I) and 36 with ratio less than 1 (type II). Immunoradiometric assay of cathepsin D in both types of cyst fluids revealed the presence of large amounts of this proteinase. The average concentration of cathepsin D in type I cyst fluids was 63.3 nmol/l, which was significantly higher than that corresponding to type II cyst fluids (35.1 nmol/l). Immunoprecipitation analysis of intracystic cathepsin D demonstrated that this protein was present as the 52 kD non-processed precursor form of the molecule. Since procathepsin D is a useful prognostic marker in breast carcinoma, we suggest that cyst fluid quantification of cathepsin D could aid to detect patients affecting of gross cystic disease with higher risk for developing breast cancer.
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Base de dados:
MEDLINE
Assunto principal:
Catepsina D
/
Exsudatos e Transudatos
/
Doença da Mama Fibrocística
/
Isoenzimas
Tipo de estudo:
Etiology_studies
Limite:
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article