The presence of a novel extracellular hyaluronidase in squid cranial cartilage.
Biochimie
; 86(8): 579-86, 2004 Aug.
Article
em En
| MEDLINE
| ID: mdl-15388235
ABSTRACT
A new type of hyaluronidase was isolated from squid cranial cartilage. The enzyme seems to be localised extracellularly, since it is extracted from the tissue by 0.5 M sodium acetate, pH 7.0, in the presence of proteinase inhibitors. Degradation studies suggest that the enzyme belongs to the family of endoglycosidases generating oligosaccharides of rather large size. The best activity of the enzyme was observed at pH 7.0 and 37 degrees C and the optimum buffer for digestion was 0.15 M Tris acetate. It is inactive in sodium phosphate, morpholine acetate and HEPES buffers. The enzyme degrades aggrecan, hyaluronan, chondroitin sulphate and oversulphated chondroitin sulphate.
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Base de dados:
MEDLINE
Assunto principal:
Crânio
/
Decapodiformes
/
Cartilagem
/
Hialuronoglucosaminidase
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article