[The function of ERK on Fas-mediated apoptotic signaling pathway of human gastric carcinoma cells].
Zhonghua Zhong Liu Za Zhi
; 27(4): 201-3, 2005 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-15949415
ABSTRACT
OBJECTIVE:
To analyze the expression and functions of ERK (extracellular regulated kinase) in Fas-mediated apoptosis in gastric carcinoma cell line SGC-7901 and to elucidate the potential significance of this signaling pathway in tumor progression.METHODS:
Radioisotope labeling and Western blotting with special anti-ERK antibody were used to check ERK activity in SGC-7901 cell line after anti-Fas antibody treatment. Apoptosis induced by several treatment factors was evaluated by FACS can flow cytometer.RESULTS:
ERK activity increased and reached the peak at 30 min after treatment with anti-Fas antibody and decreased in PD98059 pretreated group. The number of sub-G(1) cell was 30.5% +/- 2.6% in PD98059 pretreated group, which was higher than anti-Fas treatment group and control group, respectively.CONCLUSION:
In gastric cancer cell line SGC-7901, Fas-induced ERK activation may suppress Fas-mediated apoptosis. Inhibition of ERK may enhance the sensitivity of SGC-7901 cells to Fas-mediated apoptosis. Fas-induced ERK activation may confer gastric cancer cells ability to escape the immune surveillance.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Transdução de Sinais
/
Apoptose
/
MAP Quinases Reguladas por Sinal Extracelular
/
Proteína Ligante Fas
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article