One-step analysis of protein complexes in microliters of cell lysate.
Nat Methods
; 2(11): 833-5, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-16278653
ABSTRACT
We present 'mix and measure' procedures for the analysis of protein complexes in microliters of crude human and mouse cell lysates using fluorescence correlation and crosscorrelation spectroscopy. We labeled interacting endogenous proteins by indirect immunofluorescence with all primary and secondary reagents added in one step. Especially for the screening of compounds interfering with interactions that depend on signaling-induced posttranslational modifications, the approach represents a major advance over existing protocols.
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Base de dados:
MEDLINE
Assunto principal:
Análise Espectral
/
Extratos Celulares
/
Técnica Indireta de Fluorescência para Anticorpo
/
Complexos Multiproteicos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article