Quantitative analysis of clonal bone marrow CD19+ B cells: use of B cell lineage trees to delineate their role in the pathogenesis of light chain amyloidosis.
Clin Immunol
; 120(1): 106-20, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16515886
ABSTRACT
Light chain amyloidosis (AL) is a bone marrow (BM) plasma cell neoplasia with systemic deposition of Ig light chain amyloid fibrils. Here, we report the identification of clonal CD19 B cells in the BM and the use of a novel mathematical algorithm to generate B cell lineage trees of the clonal CD19 B cells and CD138 plasma cells from the BM of AL patients to delineate the relationship between these two clonal populations. The CD19+ clonal B cells in the BM of AL patients related to the clonal plasma cells represent a pre-plasma cell precursor population. The B cell lineage trees from AL patients also show significant differences in clonal diversification and antigenic selection compared to clones from normal, healthy controls. These data provide a robust example of the use of graphical quantification methods in delineating the role of neoplastic precursors in the pathogenesis of hematopoietic malignancies.
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Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Doenças da Medula Óssea
/
Glicoproteínas de Membrana
/
Linfócitos B
/
Cadeias Leves de Imunoglobulina
/
Antígenos CD19
/
Amiloidose
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article