Lactulose and mannitol intestinal permeability detected by capillary electrophoresis.
J Chromatogr B Analyt Technol Biomed Life Sci
; 834(1-2): 183-7, 2006 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-16530494
ABSTRACT
Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pK(a) and M(r). Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018-0.051) and 0.067 (0.050-0.127), respectively (p < 0.01, median, interquartile range).
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Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Absorção Intestinal
/
Lactulose
/
Manitol
Tipo de estudo:
Observational_studies
Limite:
Adolescent
/
Adult
/
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article