MAP kinase pathway-dependent phosphorylation of the L1-CAM ankyrin binding site regulates neuronal growth.
Mol Biol Cell
; 17(6): 2696-706, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16597699
ABSTRACT
The growth of neuronal processes depends critically on the function of adhesion proteins that link extracellular ligands to the cytoskeleton. The neuronal adhesion protein L1-CAM serves as a receptor for nerve growth-promoting proteins, a process that is inhibited by the interaction between L1-CAM and the cytoskeleton adaptor ankyrin. Using a novel reporter based on intramolecular bioluminescence resonance energy transfer, we have determined that the MAP kinase pathway regulates the phosphorylation of the FIGQY motif in the adhesion protein L1-CAM and its interaction with ankyrin B. MAP kinase pathway inhibitors block L1-CAM-mediated neuronal growth. However, this blockade is partially rescued by inhibitors of L1-CAM-ankyrin binding. These results demonstrate that the MAP kinase pathway regulates L1-CAM-mediated nerve growth by modulating ankyrin binding, suggesting that nerve growth can be regulated at the level of individual receptors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neuritos
/
Anquirinas
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Sistema de Sinalização das MAP Quinases
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Molécula L1 de Adesão de Célula Nervosa
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article