MMP-9 antisense oligodeoxynucleotide exerts an inhibitory effect on osteoclastic bone resorption by suppressing cell migration.
Life Sci
; 79(17): 1657-60, 2006 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-16806280
ABSTRACT
We have previously shown that matrix metalloproteinases (MMPs) play a role in osteoclastic bone resorption by facilitating migration of osteoclastic cells toward bone surface through matrices. Of MMPs identified so far, MMP-9 is likely the most important proteinase for the action, since osteoclasts express this enzyme at a tremendously high level. However, no direct evidence has been provided to demonstrate its contribution to bone resorption. In this study, to address this point, we used an MMP-9 antisense phosphothiorate oligodeoxynucleotide (S-ODN), which was shown to inhibit the protein synthesis of MMP-9 efficiently. We demonstrated that the antisense S-ODN inhibited osteoclastic pit formation on matrigel-coated dentine slices in a concentration-dependent manner with a maximum reduction of total pit volume by 53% at 10 microM. These results, taken together, suggest that MMP-9 is involved in osteoclastic bone resorption process possibly by facilitating migration of osteoclasts through proteoglican-rich matrices.
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Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Reabsorção Óssea
/
Movimento Celular
/
Oligodesoxirribonucleotídeos Antissenso
/
Metaloproteinase 9 da Matriz
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article