The control of UCP1 is dissociated from that of PGC-1alpha or of mitochondriogenesis as revealed by a study using beta-less mouse brown adipocytes in culture.
FEBS Lett
; 580(19): 4661-6, 2006 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-16876797
ABSTRACT
In rodent brown adipose tissue, the beta-adrenergic signaling is believed, by an action on PGC-1alpha, to control UCP1 expression and mitochondriogenesis. We addressed this hypothesis using beta(1)/beta(2)/beta(3)-adrenoceptor knockout (beta-less) brown adipocytes in primary culture. In these cells (a) proliferation and differentiation into multilocular cells were normal; (b) UCP1 mRNA expression was dramatically decreased (by 93%), whereas PGC-1alpha and mtTFA mRNA expressions were not; (c) UCP1, PGC-1alpha and COX IV protein expressions were decreased by 97%, 62% and 22%, respectively. Altogether the data show a dissociation between the control of UCP1, which is mostly beta-adrenoceptor-dependent and that of PGC-1alpha and of mitochondriogenesis which are not.
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Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo Marrom
/
Proteínas de Transporte
/
Transativadores
/
Receptores Adrenérgicos beta
/
Adipócitos
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article