Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning.
Anal Chem
; 78(17): 6202-14, 2006 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-16944903
ABSTRACT
We report a method for the identification and quantification of glycerophospholipid molecular species that is based on the simultaneous automated acquisition and processing of 41 precursor ion spectra, specific for acyl anions of common fatty acids moieties and several lipid class-specific fragment ions. Absolute quantification of identified species was linear within a concentration range of 10 nM-100 microM and was achieved by spiking into total lipid extracts a set of synthetic lipid standards with diheptadecanoyl (170/170) fatty acid moieties, representing six common classes of glycerophospholipids. The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome.
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MEDLINE
Assunto principal:
Glicerofosfolipídeos
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Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article