Molecular cloning and functional expression of the cardiac sarcolemmal Na(+)-Ca2+ exchanger.
Science
; 250(4980): 562-5, 1990 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-1700476
ABSTRACT
The Na(+)-Ca2+ exchanger of the cardiac sarcolemma can rapidly transport Ca2+ during excitation-contraction coupling. To begin molecular studies of this transporter, polyclonal antibodies were used to identify a complementary DNA (cDNA) clone encoding the Na(+)-Ca2+ exchanger protein. The cDNA hybridizes with a 7-kilobase RNA on a Northern blot and has an open reading frame of 970 amino acids. Hydropathy analysis suggests that the protein has multiple transmembrane helices, and a small region of the sequence is similar to that of the Na(+)- and K(+)-dependent adenosine triphosphatase. Polyclonal antibodies to a synthetic peptide from the deduced amino acid sequence react with sarcolemmal proteins of 70, 120, and 160 kilodaltons on immunoblots. RNA, synthesized from the cDNA clone, induces expression of Na(+)-Ca2+ exchange activity when injected into Xenopus oocytes.
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Base de dados:
MEDLINE
Assunto principal:
Sarcolema
/
Proteínas de Transporte
/
Expressão Gênica
/
Clonagem Molecular
/
Miocárdio
Limite:
Animals
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article