Investigation of the origin of extracellular RNA in human cell culture.
Ann N Y Acad Sci
; 1075: 50-6, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-17108191
ABSTRACT
We have used the human ECV 304 cell line to study the origin and fate of extracellular RNA (exRNA) in cell culture. Quantification of different extracellular RNA species using reverse transcription followed by quantitative PCR revealed a prevalent fraction of ribosomal RNAs. Comparison of intracellular and extracellular ribosomal RNA copy numbers allowed the calculation of the number of destroyed cells that would result in the corresponding number of extracellular rRNAs. Interestingly, this number was comparable to the amount of destroyed cells as determined by the measurement of extracellular lactate dehydrogenase activity.
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Base de dados:
MEDLINE
Assunto principal:
RNA
/
RNA Ribossômico 18S
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article