Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics.
Mol Plant Microbe Interact
; 19(12): 1385-93, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17153923
ABSTRACT
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and beta-glucuronidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root-microbe interactions.
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Base de dados:
MEDLINE
Assunto principal:
Rhizobium
/
Transformação Genética
/
Genômica
/
Phaseolus
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article