Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis.
J Proteome Res
; 7(4): 1683-92, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-18338859
ABSTRACT
Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saponinas
/
Proteínas
/
Membrana Celular
/
Permeabilidade da Membrana Celular
/
Proteômica
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article