The effect of H-ras oncogene transfection on response of mink lung epithelial cells to growth factors and cytotoxic drugs.
Anticancer Res
; 11(3): 1349-52, 1991.
Article
em En
| MEDLINE
| ID: mdl-1888171
ABSTRACT
Mink lung epithelial cells were transfected with c-myc and activated H-ras genes. The transfected sublines formed colonies in soft agar and were tumorigenic when injected subcutaneously into athymic nude mice. DNA synthesis was measured in each of the cell lines by 3H-thymidine incorporation and in the parent line there was dose related stimulation of DNA synthesis by epidermal growth factor (EGF) and inhibition by transforming growth factor-beta (TGF-beta). The c-myc transfected line had a reduced inhibitory response to TGF-beta and an exaggerated stimulatory response to EGF whereas the activated H-ras1 transfected line did not respond to TGF-beta or EGF. The activated H-ras1 transfected line was significantly more resistant to doxorubicin (ID50, 4.4 nM) and vincristine (ID50, 4.9 nM) than the parent mink lung epithelial cell line (ID50, 2.7 nM and 2.4 nM respectively). It would appear that oncogene transfection can alter the sensitivity of mink lung epithelial cells to both exogenous growth factors and cytotoxic drugs.
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Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Transformação Celular Neoplásica
/
Fator de Crescimento Transformador beta
/
Genes ras
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Fator de Crescimento Epidérmico
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Pulmão
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Antineoplásicos
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article