[Preparation and in vitro targeting of follicle stimulating hormone polypeptide modified nanoparticles].
Zhonghua Fu Chan Ke Za Zhi
; 43(7): 533-7, 2008 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-19080519
ABSTRACT
OBJECTIVE:
To prepare follicle stimulating hormone (FSH) polypeptide modified nanoparticles (NP) in order to achieve specific ovarian tumor targeting.METHODS:
Expression of FSH receptor protein in human liver cancer and ovarian cancer cell lines BEL-7402, SKOV-3 and Caov-3 was detected by immunocytochemistry. The polypeptide fragment of FSH beta 81-95 amino acids (FSHL81-95) was synthesized and covalently coupled to NP. The specific binding of FSHL81-95 and FSHL81-95-NP was examined by fluorescence microscopy and flow cytometry.RESULTS:
BEL-7402 and SKOV-3 cells were negative for FSH receptor staining, while Caov-3 cells were positive. The diameters of NP were about 100 nm, with a Zeta potential of -25 mV or so. Caov-3 cells showed a more specific interaction with FSHL81-95-NP than SKOV-3 cells (4.17 +/- 0.86 and 2.30 +/- 0.21; P < 0.05). The uptake of FSHL81-95-NP was more than NP in Caov-3 cells (4.17 +/- 0.86 and 0.41 +/- 0.32; P < 0.05). FSHL81-95-NP showed a selective targeting at Caov-3 cells compared with control NP.CONCLUSION:
FSH polypeptide modified NP could selectively target ovarian cancer cells expressing FSH receptor, which might contribute to specific endocytosis mediated by FSH receptor.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Peptídeos
/
Receptores do FSH
/
Sistemas de Liberação de Medicamentos
/
Subunidade beta do Hormônio Folículoestimulante
/
Nanopartículas
Limite:
Female
/
Humans
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article