Relative chemokine and adhesion molecule expression in Mediterranean spotted fever and African tick bite fever.
J Infect
; 58(1): 68-75, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-19091423
ABSTRACT
OBJECTIVES:
Mediterranean spotted fever (MSF) caused by Rickettsia conorii (R. conorii) is a potential lethal disease while African tick bite fever (ATBF) caused by Rickettsia africae is a self-limiting flu-like illness. We hypothesized that different inflammatory potential in endothelial cells could contribute to the different clinical features in these rickettsioses.METHODS:
We analyzed the effect of heat-inactivated R. africae and R. conorii on the mRNA and protein levels of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-8 and adhesion molecules in endothelial cells. Serum samples from patients with MSF (n=16) and ATBF (n=15) were collected before and after therapy.RESULTS:
R. conorii induced a marked increase in MCP-1, IL-8, and adhesion molecules in endothelial cells, involving toll-like receptor 4 activation. In contrast, R. africae induced MCP-1 expression, but only modest or no responses were seen on IL-8 and adhesion molecules. Comparable to the in vitro response, levels of IL-8 and adhesion molecules showed no or only a modest increase in ATBF patients while these inflammatory markers were markedly elevated during MSF.CONCLUSIONS:
Our findings suggest a superior inflammatory potential of R. conorii as compared to R. africae in endothelial cells, potentially related to the more severe inflammation in MSF comparing ATBF.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rickettsia
/
Febre Botonosa
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Moléculas de Adesão Celular
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Quimiocinas
/
Mordeduras e Picadas de Insetos
Limite:
Adult
/
Animals
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article