Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humans.
Arterioscler Thromb Vasc Biol
; 29(1): 121-7, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-19092138
ABSTRACT
OBJECTIVE:
An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function. METHODS ANDRESULTS:
Twelve sedentary subjects participating in a worksite wellness program underwent endothelial flow-mediated dilation (FMD) testing of the brachial artery and blood sampling for EPC colony assay. Microarray-based genotypic characterization of colonies showed surface markers consistent with T lymphocyte phenotype, but not with an EPC (CD34, CD133, VEGFR-2) or endothelial (CD146) phenotype. Gene expression patterns more closely matched T lymphocytes (r=0.87) than endothelial cells (r=0.66) in our microarray database. Flow cytometry of colonies confirmed large populations of CD3+CD45+ T cells (>75%) and few CD146+CD45- endothelial cells (<1%). Further, there was no correlation between colony number and the magnitude of FMD (r=-0.1512, P=0.6389). After exercise training, subjects improved FMD, from 6.7+/-2.0 to 8.7+/-1.9% (P=0.0043). Colonies also increased (P=0.0210), but without relation to FMD (r=0.1074, P=0.7396). T lymphocyte phenotype persisted after exercise (r=0.87).CONCLUSIONS:
Cells in a commonly used EPC colony assay have a gene expression and cell surface marker profile consistent with a predominance of T lymphocytes and have an unclear relevance to endothelial function, either before or after exercise training.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
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Vasodilatação
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Endotélio Vascular
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Análise de Sequência com Séries de Oligonucleotídeos
Limite:
Adult
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article