On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins.
Methods Mol Biol
; 527: 191-9, x, 2009.
Article
em En
| MEDLINE
| ID: mdl-19241014
ABSTRACT
Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Sistemas On-Line
/
Cromatografia Líquida
/
Análise de Sequência de Proteína
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article