Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples.
J Clin Microbiol
; 47(8): 2586-9, 2009 Aug.
Article
em En
| MEDLINE
| ID: mdl-19494077
ABSTRACT
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).
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Base de dados:
MEDLINE
Assunto principal:
Ruminantes
/
Reação em Cadeia da Polimerase
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Infecções por Herpesviridae
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Herpesviridae
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Transtornos Linfoproliferativos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article