[Effect of Flammulina velutipes polysaccharides on production of cytokines by murine immunocytes and serum levels of cytokines in tumor-bearing mice].
Zhong Yao Cai
; 32(4): 561-3, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19645242
ABSTRACT
OBJECTIVE:
To study the effect of Flammulina velutipes polysaccharides (FVP) on the production of tumor necrosis factor alpha (TNF-alpha), interferon-gamma (INF-gamma) and interleukin 2 (IL-2) by murine immunocytes.METHODS:
The cell's metabolic activity was determined with methylthiazolyl tetrazolium (MTT) colorimetry assay and the amounts of TNF-alpha, INF-gamma and IL-2 were measured by ELISA.RESULTS:
FVP (200, 100, 50 microg/mL) could promote the metabolic activity of murine splenocytes and peritoneal exudate cells (PEC) and increase the amounts of TNF-alpha, INF-gamma and IL-2 in the supernatants of splenocyte cultures, and the amount of TNF-alpha in PEC cultures, with the most marked increase on TNF-alpha level. FVP (100, 50, 25 mg/kg) could raise the serum levels of TNF-alpha and INF-gamma in S180 tumor-bearing mice.CONCLUSION:
FVP may regulate murine immune function through promoting the production of TNF-alpha, INF-gamma and IL-2.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Sarcoma 180
/
Baço
/
Citocinas
/
Agaricales
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article