Floxin, a resource for genetically engineering mouse ESCs.
Nat Methods
; 7(1): 50-2, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19966808
ABSTRACT
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Células-Tronco Embrionárias
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article