Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.
J Biol Chem
; 285(10): 7712-21, 2010 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-20037163
ABSTRACT
Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect in non-tumoral fibroblasts. In addition, motility (migration speed, random motility coefficient, wound healing) of the tumor cells could be decreased by the cell-permeant peptides. Although the peptides differently influenced actin polymerization in vitro, they could directly bind monomeric actin as determined by NMR and calorimetry studies. Therefore, cell-penetrating peptides might interact with intracellular protein partners, such as actin. In addition, the fact that they could reverse the tumoral phenotype is of interest for therapeutic purposes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Actinas
/
Fibroblastos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article