[Acupoint-injection of histamine induced analgesic effect in acute adjuvant-induced-arthritis rats].

ABSTRACT

OBJECTIVE: To observe the activation effect of histamine on mast cells in the acupoint area and its analgesic effects similar to acupuncture stimulation, so as to reveal the underlying mechanism of acupoint injection therapy. METHODS: A total of 60 SD rats were divided into control, model, normal saline (NS), histamine-injection and acupuncture groups (12 rats/group). Acute adjuvant-induced-arthritis (AIA) model was duplicated by injection of complete Freunds' Adjuvant (0.05 mL) into the left ankle articular cavity. For rats of saline and histamine groups, normal saline (50 microL) and histamine (50 miroL, 100 microg/mL) were injected into the left "Zusanli" (ST 36) acupoint area. For rats of acupuncture group, manual acupuncture (lifting-thrusting and twisting needle intermittently) was applied to ST 36 for 20 min. Paw withdrawal (pain threshold, PT) was detected by radiant heat irradiation of the hindpaw. Skin and muscle tissues of ST 36 area were sampled to be fixed in formalin, then, sliced (5 microm), and stained with Toluidine Blue, separately, followed by counting the degranulation rate of mast cells under microscope. RESULTS: Compared with normal control group and pre-modeling, PT of model, NS, acupuncture and histamine groups decreased significantly (P < 0.01), while in comparison with model group, PT of acupuncture and histamine groups increased considerably (P < 0.01), suggesting an induction of analgesic effect after acupuncture and histamine injection. The degranulation rates of acupuncture and histamine groups were remarkably higher than those of model and NS groups (P < 0.01), suggesting an activation of mast cells following regional acupuncture stimulation and histamine injection. CONCLUSION: Both manual acupuncture and histamine injection of ST 36 can relieve pain in AIA rats, which may be closely related to its effect in activating mast cells in acupoint area. It suggests that the mast cells are a new target for acupoint injection therapy.