Vibrio stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata.
Int J Syst Evol Microbiol
; 61(Pt 9): 2180-2185, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-20935089
ABSTRACT
A bacterial strain designated KTW-12(T) was isolated from a reef-building coral in Kenting, Taiwan, and was characterized using a polyphasic taxonomic approach. Strain KTW-12(T) was Gram-negative, semi-transparent, slightly curved rod-shaped, and non-motile. Growth occurred at 15-35 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0), and with 0.5-6.0â% NaCl (optimum, 2â%). The major cellular fatty acids were summed feature 3 (C161ω7c and/or C161ω6c), C140 and C160. The DNA G+C content was 47.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KTW-12(T) was most closely related to Vibrio porteresiae MSSRF30(T), with 94.8â% gene sequence similarity. Further multilocus sequence analysis using rpoA, recA and pyrH genes also revealed low levels of sequence similarity (74.6-85.0â%) with all species of the genus Vibrio with validly published names. A multigene phylogenetic tree using concatenated sequences of the four genes (16S rRNA, rpoA, recA and pyrH) elucidated that strain KTW-12(T) occupied a distinct phylogenetic position, forming a long branch that was not clustered with any other known species of the genus Vibrio. Strain KTW-12(T) differed from V. porteresiae MSSRF30(T) in the ability to reduce nitrate to nitrite, hydrolysis of chitin, fermentation of sorbitol and production of arginine dihydrolase, valine arylamidase, cystine arylamidase and N-acetyl-ß-glucosaminidase. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain KTW-12(T) should be classified as representing a novel species, for which the name Vibrio stylophorae sp. nov. is proposed. The type strain is KTW-12(T) (â=âBCRC 80105(T) â=âLMG 25357(T)).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vibrio
/
Antozoários
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article