Extensive role of the general regulatory factors, Abf1 and Rap1, in determining genome-wide chromatin structure in budding yeast.
Nucleic Acids Res
; 39(6): 2032-44, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21081559
ABSTRACT
The packaging of eukaryotic DNA into chromatin has profound consequences for gene regulation, as well as for other DNA transactions such as recombination, replication and repair. Understanding how this packaging is determined is consequently a pressing problem in molecular genetics. DNA sequence, chromatin remodelers and transcription factors affect chromatin structure, but the scope of these influences on genome-wide nucleosome occupancy patterns remains uncertain. Here, we use high resolution tiling arrays to examine the contributions of two general regulatory factors, Abf1 and Rap1, to nucleosome occupancy in Saccharomyces cerevisiae. These factors have each been shown to bind to a few hundred promoters, but we find here that thousands of loci show localized regions of altered nucleosome occupancy within 1 h of loss of Abf1 or Rap1 binding, and that altered chromatin structure can occur via binding sites having a wide range of affinities. These results indicate that DNA-binding transcription factors affect chromatin structure, and probably dynamics, throughout the genome to a much greater extent than previously appreciated.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Fatores de Transcrição
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Cromatina
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a Telômeros
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article