[Rat in vitro plasma-binding percentage determination of sinomenine hydrochloride by using microdialysis method and ultrafiltration].
Zhongguo Zhong Yao Za Zhi
; 35(19): 2616-9, 2010 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-21174777
ABSTRACT
OBJECTIVE:
To assess feasibility of plasma protein-binding percentage by using microdialysis method.METHOD:
Sinomenine hydrochloride was selected as model drug. The in vitro rat plasma protein-binding percentage of three kinds of concentration was determinated by using microdialysis sampling tool and HPLC-UV. At the same time the classical study method, such as ultrafiltration method, was also used to determinate the protein-binding percentage to compare the difference between the two methods.RESULT:
The in vitro rat plasma protein-binding percentage of sinomenine hydrochloride using microdialysis method was about 26% and kept relative stable at the concentration range. There was no significant difference between the two methods.CONCLUSION:
The results shows that the binding percentage is relative low. Microdialysis method is a new method for the study of protein-binding degree.
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Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Ligação Proteica
/
Ultrafiltração
/
Microdiálise
/
Morfinanos
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article