Substrate interactions during the biodegradation of BTEX and THF mixtures by Pseudomonas oleovorans DT4.
Bioresour Technol
; 102(12): 6644-9, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21511464
ABSTRACT
The efficient tetrahydrofuran (THF)-degrading bacterium, Pseudomonas oleovorans DT4 was used to investigate the substrate interactions during the aerobic biotransformation of THF and BTEX mixtures. Benzene and toluene could be utilized as growth substrates by DT4, whereas cometabolism of m-xylene, p-xylene and ethylbenzene occurred with THF. In binary mixtures, THF degradation was delayed by xylene, ethylbenzene, toluene and benzene in descending order of inhibitory effects. Conversely, benzene (or toluene) degradation was greatly enhanced by THF leading to a higher degradation rate of 39.68 mg/(h g dry weight) and a shorter complete degradation time about 21 h, possibly because THF acted as an "energy generator". Additionally, the induction experiments suggested that BTEX and THF degradation was initiated by independent and inducible enzymes. The transient intermediate hydroquinone was detected in benzene biodegradation with THF while catechol in the process without THF, suggesting that P. oleovorans DT4 possessed two distinguished benzene pathways.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tolueno
/
Xilenos
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Benzeno
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Derivados de Benzeno
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Pseudomonas oleovorans
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Misturas Complexas
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Furanos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article