A visible, targeted high-efficiency gene delivery and transfection strategy.
BMC Biotechnol
; 11: 56, 2011 May 21.
Article
em En
| MEDLINE
| ID: mdl-21600027
ABSTRACT
BACKGROUND:
To enhance myocardial angiogenic gene expression, a novel gene delivery strategy was tested. Direct intramyocardial injection of an angiogenic gene with microbubbles and insonation were applied in a dog animal model. Dogs received one of the four different treatments in conjunction with either the enhanced green fluorescence protein (EGFP) gene or the hepatocyte growth factor (HGF) gene gene with microbubbles (MB) and ultrasound (US); gene with US; gene with MB; or the gene alone.RESULTS:
Distribution of MB and the gene in the myocardium was visualized during the experiment. Compared with the EGFP gene group, an average 14.7-fold enhancement in gene expression was achieved in the EGFP+MB/US group (P < 0.01). Compared with the HGF gene group, an average 10.7-fold enhancement in gene expression was achieved in the HGF+MB/US group (P < 0.01). In addition, capillary density increased from 20.8 ± 3.4/mm2 in the HGF gene group to 146.7 ± 31.4/mm2 in HGF+MB/US group (P < 0.01).CONCLUSIONS:
Thus, direct intramyocardial injection of an angiogenic gene in conjunction with microbubbles plus insonation synergistically enhances angiogenesis. This method offers an observable gene delivery procedure with enhanced expression efficiency of the delivered gene.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transfecção
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Fator de Crescimento de Hepatócito
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Indutores da Angiogênese
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Microbolhas
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Infarto do Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article