Purification of tubulin from porcine brain.

ABSTRACT

Microtubules, polymers of the heterodimeric protein αß-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αß-tubulin. In this chapter, we describe the process of purification of heterodimeric αß-tubulin from porcine brain.