Simultaneous nitrite-dependent anaerobic methane and ammonium oxidation processes.
Appl Environ Microbiol
; 77(19): 6802-7, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21841030
ABSTRACT
Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including "Candidatus Methylomirabilis oxyfera," and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescence in situ hybridization and 16S rRNA and pmoA gene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m(3)/day (17.2 mmol day(-1)). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Compostos de Amônio Quaternário
/
Metano
/
Nitritos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article