Distal leucines are key functional determinants of Alix-binding simian immunodeficiency virus SIV(smE543) and SIV(mac239) type 3 L domains.
J Virol
; 85(21): 11532-7, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21849430
ABSTRACT
In addition to PTAP L domains, primate lentiviruses carry Alix-binding motifs that include the recently described type 3 SREKPYKEVTEDLLHLNSLF sequence. We examined the requirements for the type 3 sequence motif in simian immunodeficiency virus SIV(smE543) and identified the (499)LNSLF(503) sequence as a key functional determinant. Mutation of distal leucines (499)L and (502)L (LL mutant) caused an inhibitory effect on Alix-dependent SIV(smE543) release that was quantitatively similar to that observed following disruption of the type 3 L domain or RNA interference (RNAi) depletion of Alix. Similar results were obtained with the SIV(mac239) LL mutant. Thus, distal leucines are key determinants of SIV(smE543) and SIV(mac239) type 3 L domains.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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Proteínas de Ligação ao Cálcio
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Vírus da Imunodeficiência Símia
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Motivos de Aminoácidos
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Interações Hospedeiro-Patógeno
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Liberação de Vírus
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article