Distribution of TRPC6 in the cerebrospinal fluid-contacting nucleus of rat brain parenchyma and its expression in morphine dependence and withdrawal.
Neurochem Res
; 36(12): 2316-21, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21858483
ABSTRACT
To investigate the role of cerebrospinal fluid contacting nucleus (CSF-CN) and the changes of TRPC6 expression in morphine dependence and withdrawal. Male Sprague-Dawley rats (250 ± 50 g) were randomly divided into four groups the normal group (N); the saline group (S); the morphine dependence group (D); the morphine withdrawal group (W). All animals in each group were tested the morphine withdrawal-like behavioral signs by total withdrawal scores. Double-labeled immunofluorescent technique and laser scanning confocal microscopy were used to identify the expression of TRPC6 in CSF-CN. TRPC6 labeled neurons were found in CSF-CN and the number of CB-HRP/TRPC6 double labeled neurons in CSF-CN significantly increased. TRPC6 existed in CSF-CN of the normal rats and its expression in morphine dependence and withdrawal increased. CSF-CN might participate in the development of morphine dependence and withdrawal by the up-regulated expression of TRPC6.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Síndrome de Abstinência a Substâncias
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Substância Cinzenta Periaquedutal
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Canais de Cátion TRPC
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Morfina
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Dependência de Morfina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article