Galvanostatic entrapment of penicillinase into polytyramine films and its utilization for the potentiometric determination of penicillin.
Sensors (Basel)
; 10(4): 2851-68, 2010.
Article
em En
| MEDLINE
| ID: mdl-22319276
ABSTRACT
A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3, and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3-283 µM for penicillin and achieved a minimum detectable concentration of 0.3 µM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102±6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Penicilinase
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Potenciometria
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Técnicas Biossensoriais
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Tiramina
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Leite
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article