Two-step efficient synthesis of 5-methyluridine via two thermostable nucleoside phosphorylase from Aeropyrum pernix.
Bioorg Med Chem Lett
; 22(5): 2102-4, 2012 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-22325947
ABSTRACT
5-Methyluridine has been synthesized in high yield using guanosine and thymine as starting materials in the presence of highly thermostable recombinant purine nucleoside phosphorylase (PNP) and uridine phosphorylase (UP) obtained from hyperthermophilic aerobic crenarchaeon Aeropyrum pernix. Key reaction parameters such as pH, temperature, concentration of buffer and substrates were investigated. At the optimal conditions, 5-methyluridine was achieved in yield 85% with a guanosine conversion of 96% in 10ml scale. The process can be performed at high temperature, which will highly increase the solubility of substrates, therefore, this process is suitable for the industry application.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pentosiltransferases
/
Uridina
/
Aeropyrum
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article