Glycerol decreases the integrity of the perinuclear theca in boar sperm.
Zygote
; 21(2): 172-7, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-22475502
ABSTRACT
We evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cabeça do Espermatozoide
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Espermatozoides
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Núcleo Celular
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Crioprotetores
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Glicerol
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article