KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.
Nat Chem Biol
; 8(7): 612-4, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22660438
ABSTRACT
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Taq Polimerase
/
Pareamento de Bases
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article