4C technology: protocols and data analysis.
Methods Enzymol
; 513: 89-112, 2012.
Article
em En
| MEDLINE
| ID: mdl-22929766
ABSTRACT
Chromosome conformation capture (3C) technology and its genome-wide derivatives have revolutionized our knowledge on chromatin folding and nuclear organization. 4C-seq Technology combines 3C principles with high-throughput sequencing (4C-seq) to enable for unbiased genome-wide screens for DNA contacts made by single genomic sites of interest. Here, we discuss in detail the design, application, and data analysis of 4C-seq experiments. Based on many hundreds of different 4C-seq experiments, we define criteria to assess data quality and show how different restriction enzymes and cross-linking conditions affect results. We describe in detail the mapping strategy of 4C-seq reads and show advanced strategies for data analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Cromatina
/
Estatística como Assunto
/
Mapeamento Cromossômico
/
Análise de Sequência de DNA
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article