Robust 4C-seq data analysis to screen for regulatory DNA interactions.
Nat Methods
; 9(10): 969-72, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22961246
ABSTRACT
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article