A novel fluorescent sensor for detection of highly reactive oxygen species, and for imaging such endogenous hROS in the mitochondria of living cells.
Analyst
; 138(3): 775-8, 2013 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-23232359
ABSTRACT
A new dye, PTZ-Cy2, based on a hybrid cyaninephenothiazine platform, is described. Oxidation by highly reactive oxygen species (hROS) involves attack at the thiazine sulfur atom and destruction of the p conjugation of the cyaninemoiety. Dual fluorescence emissions are enhanced dramatically at λ(em) 470 nm and 595 nm, and PTZ-Cy2 thus serves both as an absorbance ratiometric and a fluorescent "off-on" sensor for detecting hROS. Moreover PTZ-Cy2 shows selectivity for hROS over other oxidants, and gives a highly sensitive response to such endogenous species within the mitochondria of living cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espécies Reativas de Oxigênio
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Microscopia Confocal
/
Corantes Fluorescentes
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Mitocôndrias
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article