[Modified montmorillonite as multifunction gene and drug delivery system].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 41(6): 631-8, 2012 11.
Article
em Zh
| MEDLINE
| ID: mdl-23239654
ABSTRACT
OBJECTIVE:
To develop polyethylenimine-Doxorubicin-montmorillonite (PEI-Dox-MTT) as a novel multifunction delivery system.METHODS:
Dox was intercalated into montmorillonite, PEI covered to the surface of Dox/MMT to make the nano-particle. XRD, FT-IR and TGA were used to confirm chemical property of the nano-particle. SEM was used to observe the morphology. The capability of drug release was investigated by PBS buffer solution (pH 7.4). The DNA binding ability of nano-particle was detected by gel electrophoresis retardation assay. The cell viability in COS-7 and SKOV3 cell lines was tested using MTT assay. The gastric mucosa protection was evaluated in vitro.RESULTS:
XRD image showed that Dox was intercalated into montmorillonite, inter space of which increased to 31.3Å; the FT-IR spectra showed the vibration bands of PEI at 1 560 cm(-1) and 2 850 cm(-1), the vibration band of Dox at 1 350 cm(-1). Size analysis and SEM revealed that the size of nano-particle was 600 nm, and the zeta-potential was 30 mV. Drug release experiment explored that the nano-particle stably released drug in range of 6 X10(-4) â 8 X10(-4) mg/ml within 72 h. MTT assay showed that the cell viability was over 80% in experiment condition in COS-7 and SKOV3 cell lines. 0.3 mg PEI-MMT nano-particle was able to protect gastric mucosa from alcohol.CONCLUSION:
Multifunction system of PEI/Dox/MMT has been prepared successfully.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Polietilenoimina
/
Bentonita
/
Sistemas de Liberação de Medicamentos
/
Vetores Genéticos
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article