[2'-Modified oligoribonucleotides, containing 1,2-diol and aldehyde groups. Synthesis and properties].
Bioorg Khim
; 38(5): 555-68, 2012.
Article
em Ru
| MEDLINE
| ID: mdl-23342489
ABSTRACT
1,2-Diol-oligoribonucleotides were prepared using fully protected 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxoethyl]uridine 3'-phosphoramidite. Incorporation of the 2'-modified uridine residue into oligonucleotide chains does not significantly affect the thermal stability of RNA and RNA-DNA duplexes. Periodate oxidation of the 1,2-diol results in reactive 2'-aldehyde oligoribonucleotides. Further application of these oligonucleotides for cross-linking with bacterial ribonuclease P was investigated.
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Base de dados:
MEDLINE
Assunto principal:
Oligorribonucleotídeos
/
DNA
/
RNA
/
Aldeídos
/
Ácidos Nucleicos Heteroduplexes
Idioma:
Ru
Ano de publicação:
2012
Tipo de documento:
Article