Reversal effect of rosmarinic acid on multidrug resistance in SGC7901/Adr cell.
J Asian Nat Prod Res
; 15(3): 276-85, 2013.
Article
em En
| MEDLINE
| ID: mdl-23421517
ABSTRACT
Multidrug resistance (MDR) has been a major problem in cancer chemotherapy. In this study, the aim was to explore the reversal effect and its potential mechanism of rosmarinic acid (RA) on SGC7901/Adr cells. 3-(4,5-Dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to investigate the reversal index of RA in SGC7901/Adr cell line. The intracellular accumulation of adriamycin, rhodamine123 (Rh123), and the expression of P-glycoprotein (P-gp) were assayed by flow cytometry. The influence of RA on the transcription of MDR1 gene was determined by reverse transcription-polymerase chain reaction. The results showed that RA could reverse the MDR of SGC7901/Adr cells, increase the intracellular accumulation of Adr and Rh123, and decrease the transcription of MDR1 gene and the expression of P-gp in SGC7901/Adr cells. These results indicated that RA was a potential multidrug resistance-reversing agent and warranted further investigations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cinamatos
/
Resistência a Múltiplos Medicamentos
/
Depsídeos
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article